UV Visible Spectrophotometry (UV-Vis)
Varian Cary 50Bio
Model: Varian Cary 50Bio
Location: 014 Brauer Hall
Description: UV –Vis spectroscopy is an analytical technique that measures the amount of discrete wavelength of UV or Visible light that are abosorbed by or transmitted through a sample in comparison to a reference or blank sample.
- Dual beam, Czerny-Turner monochromator.
- 190-1100 nm wavelength with exceptionally long life, dual Si diode detectors, quartz over coated optics.
- Non – measurement phase stepping wavelength drive, room light immunity, and central control by PC with Microsoft windows operating system.
- Measurement the concentration of a molecule in solution, observe structural or conformational transitions, measure color, monitor reaction kinetics, etc.
- Quickly verifying the purity and concentration of RNA and DNA is one particularly widespread application.
- Processing UV-Vis spectra using mathematical derivatives allows overlapping absorbance peaks in the original spectra to be resolved to identify individual pharmaceutical compounds.
- UV-Vis spectroscopy is often used in bacterial culturing to estimate the cell concentration and to track growth.
- In wastewater treatments, UV-Vis spectroscopy can be used in kinetic and monitoring studies to ensure certain dyes or dye by‑products have been removed properly by comparing their spectra over time
|Per hour rate||Trained user||By our service|
|Other departments at WUSTL||$10||$70|