UV Visible Spectrophotometry (UV-Vis)

Model: Varian Cary 50Bio

Location: 014 Brauer Hall

Description: UV –Vis spectroscopy is an analytical technique that measures the amount of discrete wavelength of UV or Visible light that are abosorbed by or transmitted through a sample in comparison to a reference or blank sample.

Capabilities:

• Dual beam, Czerny-Turner monochromator.
• 190-1100 nm wavelength with exceptionally long life, dual Si diode detectors, quartz over coated optics.
• Non – measurement phase stepping wavelength drive, room light immunity, and central control by PC with Microsoft windows operating system.

Typical uses:

• Measurement the concentration of a molecule in solution, observe structural or conformational transitions, measure color, monitor reaction kinetics, etc.
• Quickly verifying the purity and concentration of RNA and DNA is one particularly widespread application.
• Processing UV-Vis spectra using mathematical derivatives allows overlapping absorbance peaks in the original spectra to be resolved to identify individual pharmaceutical compounds.
• UV-Vis spectroscopy is often used in bacterial culturing to estimate the cell concentration and to track growth.
• In wastewater treatments, UV-Vis spectroscopy can be used in kinetic and monitoring studies to ensure certain dyes or dye by‑products have been removed properly by comparing their spectra over time

Fees

Per hour rate	Trained user	By our service
Other departments at WUSTL	$12	$70
Other universities	$20	$80
Industry Corporate	$40	$160