Digital PCR

Model: QIAcuity One

Location: Whitaker Hall 075H

Description:

The QIAGEN Digital PCR is a cutting-edge technology designed for precise and sensitive quantification of nucleic acids. Unlike traditional PCR, which offers relative quantification, Digital PCR (dPCR) achieves absolute quantification by partitioning a sample into thousands of individual reactions. Each partition is analyzed independently, and the number of positive reactions is used to determine the exact number of target molecules in the original sample. This approach is particularly effective for detecting low-abundance targets and assessing gene expression with exceptional accuracy.

Capabilities:

• Provides absolute quantification of nucleic acids with high precision and sensitivity.
• Detects low-abundance targets and rare mutations effectively.
• Eliminates the need for standard curves, reducing variability and improving accuracy.
• Handles a wide range of sample types, including DNA and RNA from various sources.

Typical Uses:

• Applied in research for precise quantification of gene expression and rare genetic variants.
• Utilized in clinical research for monitoring disease biomarkers and assessing treatment efficacy.
• Valuable for studies involving low-abundance targets, such as early cancer detection or microbial quantification.
• Supports development and validation of diagnostic assays in genomics and molecular biology.